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Sample GSM550794 Query DataSets for GSM550794
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 3
Sample type RNA
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 71
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 583.57568
AFFX-BioB-M_at 899.28845
AFFX-BioB-3_at 862.89862
AFFX-BioC-5_at 1710.87158
AFFX-BioC-3_at 1240.58313
AFFX-BioDn-5_at 2762.71875
AFFX-BioDn-3_at 4860.18994
AFFX-CreX-5_at 7232.38623
AFFX-CreX-3_at 7816.67041
AFFX-DapX-5_at 35.00836
AFFX-DapX-M_at 6.59795
AFFX-DapX-3_at 24.23842
AFFX-LysX-5_at 15.79361
AFFX-LysX-M_at 13.46181
AFFX-LysX-3_at 4.32401
AFFX-PheX-5_at 19.44931
AFFX-PheX-M_at 12.98845
AFFX-PheX-3_at 26.20999
AFFX-ThrX-5_at 6.09944
AFFX-ThrX-M_at 14.60408

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.

Supplementary file Size Download File type/resource
GSM550794.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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