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Sample GSM550797 Query DataSets for GSM550797
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 9
Sample type RNA
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 65
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 596.13336
AFFX-BioB-M_at 947.43561
AFFX-BioB-3_at 891.12189
AFFX-BioC-5_at 1849.79761
AFFX-BioC-3_at 1385.15271
AFFX-BioDn-5_at 3138.58105
AFFX-BioDn-3_at 5240.82617
AFFX-CreX-5_at 7567.83008
AFFX-CreX-3_at 8890.5
AFFX-DapX-5_at 32.78878
AFFX-DapX-M_at 6.62489
AFFX-DapX-3_at 23.15058
AFFX-LysX-5_at 8.03192
AFFX-LysX-M_at 6.94635
AFFX-LysX-3_at 7.77749
AFFX-PheX-5_at 22.48375
AFFX-PheX-M_at 14.45467
AFFX-PheX-3_at 23.07694
AFFX-ThrX-5_at 6.9729
AFFX-ThrX-M_at 22.29247

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.

Supplementary file Size Download File type/resource
GSM550797.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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