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Sample GSM550798 Query DataSets for GSM550798
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 5
Sample type RNA
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 62
gender: F
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 1008.37457
AFFX-BioB-M_at 1461.47449
AFFX-BioB-3_at 1355.76514
AFFX-BioC-5_at 2495.15161
AFFX-BioC-3_at 1908.32629
AFFX-BioDn-5_at 3482.94971
AFFX-BioDn-3_at 5848.72949
AFFX-CreX-5_at 7956.46094
AFFX-CreX-3_at 9388.5
AFFX-DapX-5_at 28.90988
AFFX-DapX-M_at 7.88749
AFFX-DapX-3_at 13.96466
AFFX-LysX-5_at 15.22874
AFFX-LysX-M_at 11.85258
AFFX-LysX-3_at 17.65431
AFFX-PheX-5_at 14.34281
AFFX-PheX-M_at 12.13548
AFFX-PheX-3_at 30.44373
AFFX-ThrX-5_at 15.66494
AFFX-ThrX-M_at 22.00652

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.

Supplementary file Size Download File type/resource
GSM550798.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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