NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM550802 Query DataSets for GSM550802
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 1
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 60
gender: M
gold stage: Stage IV
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 623.6759
AFFX-BioB-M_at 974.60205
AFFX-BioB-3_at 901.8454
AFFX-BioC-5_at 1972.06042
AFFX-BioC-3_at 1434.02844
AFFX-BioDn-5_at 3169.80493
AFFX-BioDn-3_at 5571.07764
AFFX-CreX-5_at 7780.81104
AFFX-CreX-3_at 9022.95996
AFFX-DapX-5_at 39.55539
AFFX-DapX-M_at 4.25596
AFFX-DapX-3_at 13.63025
AFFX-LysX-5_at 13.63671
AFFX-LysX-M_at 5.17497
AFFX-LysX-3_at 12.8154
AFFX-PheX-5_at 22.89116
AFFX-PheX-M_at 15.37252
AFFX-PheX-3_at 25.17048
AFFX-ThrX-5_at 10.04525
AFFX-ThrX-M_at 12.96753

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550802.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap