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Sample GSM550803 Query DataSets for GSM550803
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 6
Sample type RNA
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 67
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 1018.32092
AFFX-BioB-M_at 1532.96765
AFFX-BioB-3_at 1460.57202
AFFX-BioC-5_at 2646.27271
AFFX-BioC-3_at 2072.78687
AFFX-BioDn-5_at 3654.31494
AFFX-BioDn-3_at 6126.02197
AFFX-CreX-5_at 7960.26416
AFFX-CreX-3_at 9680.90527
AFFX-DapX-5_at 35.65753
AFFX-DapX-M_at 8.73049
AFFX-DapX-3_at 26.56137
AFFX-LysX-5_at 20.10866
AFFX-LysX-M_at 5.76408
AFFX-LysX-3_at 16.5805
AFFX-PheX-5_at 11.09504
AFFX-PheX-M_at 16.76859
AFFX-PheX-3_at 28.4876
AFFX-ThrX-5_at 1.86972
AFFX-ThrX-M_at 30.42755

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.

Supplementary file Size Download File type/resource
GSM550803.CEL.gz 4.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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