NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM550804 Query DataSets for GSM550804
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 13
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 68
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: F
hyb date: 39324
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 642.74304
AFFX-BioB-M_at 975.92572
AFFX-BioB-3_at 876.53241
AFFX-BioC-5_at 1888.24487
AFFX-BioC-3_at 1420.30371
AFFX-BioDn-5_at 3141.93457
AFFX-BioDn-3_at 4902.34277
AFFX-CreX-5_at 7652.34082
AFFX-CreX-3_at 8325.66016
AFFX-DapX-5_at 44.17423
AFFX-DapX-M_at 3.5827
AFFX-DapX-3_at 23.74711
AFFX-LysX-5_at 9.44544
AFFX-LysX-M_at 16.16745
AFFX-LysX-3_at 6.34111
AFFX-PheX-5_at 20.4431
AFFX-PheX-M_at 8.19546
AFFX-PheX-3_at 17.10691
AFFX-ThrX-5_at 5.72804
AFFX-ThrX-M_at 13.60572

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550804.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap