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Sample GSM550806 Query DataSets for GSM550806
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 14
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 67
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 725.60315
AFFX-BioB-M_at 1118.02917
AFFX-BioB-3_at 1076.79126
AFFX-BioC-5_at 2147.37158
AFFX-BioC-3_at 1652.22351
AFFX-BioDn-5_at 3305.0874
AFFX-BioDn-3_at 5468.73828
AFFX-CreX-5_at 7961.7666
AFFX-CreX-3_at 8928.49219
AFFX-DapX-5_at 42.44368
AFFX-DapX-M_at 4.47155
AFFX-DapX-3_at 23.86668
AFFX-LysX-5_at 8.12464
AFFX-LysX-M_at 10.06354
AFFX-LysX-3_at 9.90299
AFFX-PheX-5_at 16.33642
AFFX-PheX-M_at 16.56006
AFFX-PheX-3_at 18.58127
AFFX-ThrX-5_at 13.1251
AFFX-ThrX-M_at 18.96929

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550806.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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