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Sample GSM550810 Query DataSets for GSM550810
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 18
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 70
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 541.11792
AFFX-BioB-M_at 897.46869
AFFX-BioB-3_at 825.7251
AFFX-BioC-5_at 1823.10144
AFFX-BioC-3_at 1269.23535
AFFX-BioDn-5_at 2807.08496
AFFX-BioDn-3_at 5193.94385
AFFX-CreX-5_at 7018.75439
AFFX-CreX-3_at 8240.55859
AFFX-DapX-5_at 29.22567
AFFX-DapX-M_at 9.78587
AFFX-DapX-3_at 20.44543
AFFX-LysX-5_at 13.0614
AFFX-LysX-M_at 6.69394
AFFX-LysX-3_at 8.08419
AFFX-PheX-5_at 26.23475
AFFX-PheX-M_at 14.98768
AFFX-PheX-3_at 27.53213
AFFX-ThrX-5_at 13.46281
AFFX-ThrX-M_at 14.35524

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550810.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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