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Sample GSM550811 Query DataSets for GSM550811
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 19
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 67
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 318.15567
AFFX-BioB-M_at 483.73178
AFFX-BioB-3_at 467.82297
AFFX-BioC-5_at 1028.7915
AFFX-BioC-3_at 779.43768
AFFX-BioDn-5_at 1727.74683
AFFX-BioDn-3_at 2717.30981
AFFX-CreX-5_at 4478.19336
AFFX-CreX-3_at 4717.21582
AFFX-DapX-5_at 23.81068
AFFX-DapX-M_at 7.09277
AFFX-DapX-3_at 13.96449
AFFX-LysX-5_at 17.10851
AFFX-LysX-M_at 0.79212
AFFX-LysX-3_at 12.74599
AFFX-PheX-5_at 18.36967
AFFX-PheX-M_at 13.59297
AFFX-PheX-3_at 28.25197
AFFX-ThrX-5_at 16.97532
AFFX-ThrX-M_at 12.56776

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550811.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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