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Sample GSM550813 Query DataSets for GSM550813
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 20
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 63
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: F
hyb date: 39324
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 808.89502
AFFX-BioB-M_at 1256.27039
AFFX-BioB-3_at 1213.79773
AFFX-BioC-5_at 2341.46899
AFFX-BioC-3_at 1850.41406
AFFX-BioDn-5_at 3611.93677
AFFX-BioDn-3_at 5552.8252
AFFX-CreX-5_at 7963.39502
AFFX-CreX-3_at 9537.08789
AFFX-DapX-5_at 31.38361
AFFX-DapX-M_at 8.12262
AFFX-DapX-3_at 20.41039
AFFX-LysX-5_at 13.58196
AFFX-LysX-M_at 4.37161
AFFX-LysX-3_at 9.78273
AFFX-PheX-5_at 12.56795
AFFX-PheX-M_at 16.43597
AFFX-PheX-3_at 29.30599
AFFX-ThrX-5_at 11.2835
AFFX-ThrX-M_at 20.14219

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550813.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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