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Sample GSM550814 Query DataSets for GSM550814
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 21
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 73
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: F
hyb date: 39324
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 603.26971
AFFX-BioB-M_at 965.78296
AFFX-BioB-3_at 904.04742
AFFX-BioC-5_at 1890.42749
AFFX-BioC-3_at 1402.89185
AFFX-BioDn-5_at 3243.84961
AFFX-BioDn-3_at 5210.48633
AFFX-CreX-5_at 7483.30859
AFFX-CreX-3_at 8869.97656
AFFX-DapX-5_at 42.37551
AFFX-DapX-M_at 10.12155
AFFX-DapX-3_at 14.73347
AFFX-LysX-5_at 11.5588
AFFX-LysX-M_at 10.59061
AFFX-LysX-3_at 9.75143
AFFX-PheX-5_at 12.41823
AFFX-PheX-M_at 12.65326
AFFX-PheX-3_at 20.61856
AFFX-ThrX-5_at 11.56087
AFFX-ThrX-M_at 14.50059

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550814.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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