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Sample GSM550815 Query DataSets for GSM550815
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 22
Sample type RNA
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 56
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 620.32355
AFFX-BioB-M_at 1038.27075
AFFX-BioB-3_at 904.31506
AFFX-BioC-5_at 1919.49866
AFFX-BioC-3_at 1363.19678
AFFX-BioDn-5_at 2885.56006
AFFX-BioDn-3_at 5153.59375
AFFX-CreX-5_at 6795.84863
AFFX-CreX-3_at 7933.84619
AFFX-DapX-5_at 57.11527
AFFX-DapX-M_at 11.2129
AFFX-DapX-3_at 27.27008
AFFX-LysX-5_at 9.34579
AFFX-LysX-M_at 12.00278
AFFX-LysX-3_at 14.94908
AFFX-PheX-5_at 27.69353
AFFX-PheX-M_at 12.46433
AFFX-PheX-3_at 23.91598
AFFX-ThrX-5_at 7.36183
AFFX-ThrX-M_at 23.73465

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.

Supplementary file Size Download File type/resource
GSM550815.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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