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Sample GSM550817 Query DataSets for GSM550817
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 24
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 66
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 705.6651
AFFX-BioB-M_at 1082.46289
AFFX-BioB-3_at 974.72491
AFFX-BioC-5_at 2103.11279
AFFX-BioC-3_at 1582.11047
AFFX-BioDn-5_at 3423.61401
AFFX-BioDn-3_at 5432.11621
AFFX-CreX-5_at 8341.67188
AFFX-CreX-3_at 9162.65234
AFFX-DapX-5_at 49.68063
AFFX-DapX-M_at 7.403
AFFX-DapX-3_at 23.23769
AFFX-LysX-5_at 12.93991
AFFX-LysX-M_at 4.53735
AFFX-LysX-3_at 10.28466
AFFX-PheX-5_at 17.7051
AFFX-PheX-M_at 18.68038
AFFX-PheX-3_at 28.00371
AFFX-ThrX-5_at 23.37535
AFFX-ThrX-M_at 18.26

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550817.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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