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Sample GSM550818 Query DataSets for GSM550818
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 25
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 67
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 685.3913
AFFX-BioB-M_at 1005.133
AFFX-BioB-3_at 909.50348
AFFX-BioC-5_at 1988.11499
AFFX-BioC-3_at 1468.56604
AFFX-BioDn-5_at 3343.55005
AFFX-BioDn-3_at 5547.25488
AFFX-CreX-5_at 8230.31836
AFFX-CreX-3_at 9060.34082
AFFX-DapX-5_at 35.50471
AFFX-DapX-M_at 8.64018
AFFX-DapX-3_at 25.89132
AFFX-LysX-5_at 14.17215
AFFX-LysX-M_at 1.68942
AFFX-LysX-3_at 2.68903
AFFX-PheX-5_at 16.04888
AFFX-PheX-M_at 9.99866
AFFX-PheX-3_at 19.77577
AFFX-ThrX-5_at 5.112
AFFX-ThrX-M_at 6.54274

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550818.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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