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Sample GSM550819 Query DataSets for GSM550819
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 26
Sample type RNA
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 66
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 595.73956
AFFX-BioB-M_at 850.50464
AFFX-BioB-3_at 764.95599
AFFX-BioC-5_at 1796.4082
AFFX-BioC-3_at 1353.776
AFFX-BioDn-5_at 3308.99805
AFFX-BioDn-3_at 4949.48193
AFFX-CreX-5_at 8366.5166
AFFX-CreX-3_at 8716.09668
AFFX-DapX-5_at 49.33239
AFFX-DapX-M_at 4.50078
AFFX-DapX-3_at 25.11102
AFFX-LysX-5_at 10.57471
AFFX-LysX-M_at 2.84833
AFFX-LysX-3_at 12.61312
AFFX-PheX-5_at 14.2713
AFFX-PheX-M_at 12.24521
AFFX-PheX-3_at 35.39749
AFFX-ThrX-5_at 5.29458
AFFX-ThrX-M_at 13.29054

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.

Supplementary file Size Download File type/resource
GSM550819.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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