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Sample GSM550820 Query DataSets for GSM550820
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 27
Sample type RNA
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 70
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 762.72455
AFFX-BioB-M_at 1124.57727
AFFX-BioB-3_at 974.70599
AFFX-BioC-5_at 2140.1333
AFFX-BioC-3_at 1603.07141
AFFX-BioDn-5_at 3399.31812
AFFX-BioDn-3_at 5173.68164
AFFX-CreX-5_at 8165.65234
AFFX-CreX-3_at 8705.04395
AFFX-DapX-5_at 44.84749
AFFX-DapX-M_at 6.24983
AFFX-DapX-3_at 25.08924
AFFX-LysX-5_at 11.79152
AFFX-LysX-M_at 15.51422
AFFX-LysX-3_at 11.15028
AFFX-PheX-5_at 12.13165
AFFX-PheX-M_at 14.98461
AFFX-PheX-3_at 35.33443
AFFX-ThrX-5_at 4.11163
AFFX-ThrX-M_at 15.27748

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.

Supplementary file Size Download File type/resource
GSM550820.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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