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Sample GSM550823 Query DataSets for GSM550823
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 8
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 64
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 740.24017
AFFX-BioB-M_at 1068.68872
AFFX-BioB-3_at 981.10114
AFFX-BioC-5_at 2114.44653
AFFX-BioC-3_at 1601.85803
AFFX-BioDn-5_at 3581.96216
AFFX-BioDn-3_at 5211.64697
AFFX-CreX-5_at 8346.86621
AFFX-CreX-3_at 8822.90527
AFFX-DapX-5_at 40.39361
AFFX-DapX-M_at 6.42311
AFFX-DapX-3_at 21.35675
AFFX-LysX-5_at 8.66916
AFFX-LysX-M_at 11.39563
AFFX-LysX-3_at 13.58157
AFFX-PheX-5_at 22.27452
AFFX-PheX-M_at 13.78635
AFFX-PheX-3_at 25.10788
AFFX-ThrX-5_at 6.74837
AFFX-ThrX-M_at 19.23808

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550823.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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