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Sample GSM550826 Query DataSets for GSM550826
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 30
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 64
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 893.30945
AFFX-BioB-M_at 1300.59326
AFFX-BioB-3_at 1223.31848
AFFX-BioC-5_at 2410.59766
AFFX-BioC-3_at 1867.54175
AFFX-BioDn-5_at 3795.7854
AFFX-BioDn-3_at 5823.05615
AFFX-CreX-5_at 8518.4707
AFFX-CreX-3_at 9841.99805
AFFX-DapX-5_at 30.76424
AFFX-DapX-M_at 6.33871
AFFX-DapX-3_at 22.02969
AFFX-LysX-5_at 11.29537
AFFX-LysX-M_at 9.15803
AFFX-LysX-3_at 9.30216
AFFX-PheX-5_at 8.09973
AFFX-PheX-M_at 23.56595
AFFX-PheX-3_at 27.22517
AFFX-ThrX-5_at 11.35788
AFFX-ThrX-M_at 16.54127

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550826.CEL.gz 4.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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