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Sample GSM550827 Query DataSets for GSM550827
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 11
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 60
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 545.46497
AFFX-BioB-M_at 796.46442
AFFX-BioB-3_at 734.06763
AFFX-BioC-5_at 1620.5094
AFFX-BioC-3_at 835.35809
AFFX-BioDn-5_at 2907.53906
AFFX-BioDn-3_at 4568.41553
AFFX-CreX-5_at 7748.57178
AFFX-CreX-3_at 5018.68799
AFFX-DapX-5_at 50.40272
AFFX-DapX-M_at 8.28857
AFFX-DapX-3_at 17.85539
AFFX-LysX-5_at 7.21297
AFFX-LysX-M_at 7.03752
AFFX-LysX-3_at 10.63516
AFFX-PheX-5_at 11.42437
AFFX-PheX-M_at 9.23562
AFFX-PheX-3_at 23.79806
AFFX-ThrX-5_at 8.54216
AFFX-ThrX-M_at 5.36635

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550827.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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