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Sample GSM550828 Query DataSets for GSM550828
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 12
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 68
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 902.46124
AFFX-BioB-M_at 1333.24316
AFFX-BioB-3_at 1224.82678
AFFX-BioC-5_at 2427.81689
AFFX-BioC-3_at 1865.68701
AFFX-BioDn-5_at 3788.25708
AFFX-BioDn-3_at 5535.6499
AFFX-CreX-5_at 7971.29102
AFFX-CreX-3_at 9062.31152
AFFX-DapX-5_at 44.98438
AFFX-DapX-M_at 8.59867
AFFX-DapX-3_at 21.42116
AFFX-LysX-5_at 16.05339
AFFX-LysX-M_at 0.98784
AFFX-LysX-3_at 18.82462
AFFX-PheX-5_at 16.94952
AFFX-PheX-M_at 14.39864
AFFX-PheX-3_at 26.103
AFFX-ThrX-5_at 7.44877
AFFX-ThrX-M_at 14.13001

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550828.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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