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Sample GSM550830 Query DataSets for GSM550830
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 14
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 69
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 605.59485
AFFX-BioB-M_at 936.19177
AFFX-BioB-3_at 881.5799
AFFX-BioC-5_at 1917.92102
AFFX-BioC-3_at 1389.9209
AFFX-BioDn-5_at 3372.27759
AFFX-BioDn-3_at 5409.52295
AFFX-CreX-5_at 7957.50977
AFFX-CreX-3_at 8880.44922
AFFX-DapX-5_at 34.39399
AFFX-DapX-M_at 6.92135
AFFX-DapX-3_at 16.99658
AFFX-LysX-5_at 14.64009
AFFX-LysX-M_at 7.3614
AFFX-LysX-3_at 8.38954
AFFX-PheX-5_at 10.81588
AFFX-PheX-M_at 13.27719
AFFX-PheX-3_at 18.88667
AFFX-ThrX-5_at 13.12869
AFFX-ThrX-M_at 17.62735

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550830.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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