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Sample GSM550831 Query DataSets for GSM550831
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 3
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 72
gender: M
gold stage: Stage IV
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 927.35846
AFFX-BioB-M_at 1492.25562
AFFX-BioB-3_at 1396.11194
AFFX-BioC-5_at 2514.32471
AFFX-BioC-3_at 1869.48682
AFFX-BioDn-5_at 3716.12427
AFFX-BioDn-3_at 6155.99854
AFFX-CreX-5_at 8020.16602
AFFX-CreX-3_at 9810.04785
AFFX-DapX-5_at 60.44885
AFFX-DapX-M_at 9.2821
AFFX-DapX-3_at 23.78212
AFFX-LysX-5_at 8.71532
AFFX-LysX-M_at 12.76883
AFFX-LysX-3_at 20.24643
AFFX-PheX-5_at 16.6737
AFFX-PheX-M_at 12.11707
AFFX-PheX-3_at 25.05159
AFFX-ThrX-5_at 17.02856
AFFX-ThrX-M_at 31.35462

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550831.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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