NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM550832 Query DataSets for GSM550832
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 31
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 63
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: F
hyb date: 39324
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 749.91534
AFFX-BioB-M_at 1099.16333
AFFX-BioB-3_at 1064.90405
AFFX-BioC-5_at 2236.26416
AFFX-BioC-3_at 1706.84851
AFFX-BioDn-5_at 3744.61816
AFFX-BioDn-3_at 5548.34912
AFFX-CreX-5_at 8294.06543
AFFX-CreX-3_at 9235.98242
AFFX-DapX-5_at 33.08308
AFFX-DapX-M_at 7.13277
AFFX-DapX-3_at 19.32956
AFFX-LysX-5_at 10.20133
AFFX-LysX-M_at 5.87893
AFFX-LysX-3_at 9.50878
AFFX-PheX-5_at 20.81369
AFFX-PheX-M_at 18.56765
AFFX-PheX-3_at 25.18224
AFFX-ThrX-5_at 7.02503
AFFX-ThrX-M_at 20.13144

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550832.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap