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Sample GSM550833 Query DataSets for GSM550833
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 32
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 49
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 552.83105
AFFX-BioB-M_at 975.33252
AFFX-BioB-3_at 911.78369
AFFX-BioC-5_at 1974.55957
AFFX-BioC-3_at 1390.02271
AFFX-BioDn-5_at 3332.18311
AFFX-BioDn-3_at 5758.94873
AFFX-CreX-5_at 8006.14404
AFFX-CreX-3_at 9441.9541
AFFX-DapX-5_at 49.37528
AFFX-DapX-M_at 7.59988
AFFX-DapX-3_at 25.23152
AFFX-LysX-5_at 6.45476
AFFX-LysX-M_at 5.94656
AFFX-LysX-3_at 8.33565
AFFX-PheX-5_at 13.20621
AFFX-PheX-M_at 14.44753
AFFX-PheX-3_at 24.31742
AFFX-ThrX-5_at 10.60148
AFFX-ThrX-M_at 15.6743

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550833.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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