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Sample GSM550834 Query DataSets for GSM550834
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 15
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 68
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 682.60437
AFFX-BioB-M_at 1058.49536
AFFX-BioB-3_at 953.49628
AFFX-BioC-5_at 2068.0564
AFFX-BioC-3_at 1467.97888
AFFX-BioDn-5_at 3067.14185
AFFX-BioDn-3_at 5519.63623
AFFX-CreX-5_at 7996.23438
AFFX-CreX-3_at 9176.01465
AFFX-DapX-5_at 40.03693
AFFX-DapX-M_at 11.80032
AFFX-DapX-3_at 22.72061
AFFX-LysX-5_at 11.21475
AFFX-LysX-M_at 4.88125
AFFX-LysX-3_at 12.06186
AFFX-PheX-5_at 18.01199
AFFX-PheX-M_at 20.43435
AFFX-PheX-3_at 29.84554
AFFX-ThrX-5_at 9.68028
AFFX-ThrX-M_at 29.37374

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550834.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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