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Sample GSM550835 Query DataSets for GSM550835
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 33
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 67
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 595.39508
AFFX-BioB-M_at 966.04962
AFFX-BioB-3_at 869.18494
AFFX-BioC-5_at 1955.85107
AFFX-BioC-3_at 1425.79773
AFFX-BioDn-5_at 3246.85669
AFFX-BioDn-3_at 5662.11914
AFFX-CreX-5_at 8403.19629
AFFX-CreX-3_at 9168.33496
AFFX-DapX-5_at 41.98994
AFFX-DapX-M_at 13.14991
AFFX-DapX-3_at 29.8579
AFFX-LysX-5_at 10.44716
AFFX-LysX-M_at 5.20265
AFFX-LysX-3_at 7.97845
AFFX-PheX-5_at 13.75695
AFFX-PheX-M_at 13.13454
AFFX-PheX-3_at 24.55481
AFFX-ThrX-5_at 14.36549
AFFX-ThrX-M_at 15.69613

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550835.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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