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Sample GSM550836 Query DataSets for GSM550836
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 34
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 63
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1135.33252
AFFX-BioB-M_at 1681.54822
AFFX-BioB-3_at 1546.88867
AFFX-BioC-5_at 2862.79761
AFFX-BioC-3_at 2264.81421
AFFX-BioDn-5_at 4471.62402
AFFX-BioDn-3_at 6979.19971
AFFX-CreX-5_at 9565.67383
AFFX-CreX-3_at 11071.14551
AFFX-DapX-5_at 37.04128
AFFX-DapX-M_at 9.65337
AFFX-DapX-3_at 28.18505
AFFX-LysX-5_at 11.45204
AFFX-LysX-M_at 6.52363
AFFX-LysX-3_at 13.05717
AFFX-PheX-5_at 14.95398
AFFX-PheX-M_at 28.26861
AFFX-PheX-3_at 28.29734
AFFX-ThrX-5_at 15.91231
AFFX-ThrX-M_at 24.44346

Total number of rows: 54675

Table truncated, full table size 1069 Kbytes.




Supplementary file Size Download File type/resource
GSM550836.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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