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Sample GSM550837 Query DataSets for GSM550837
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 35
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 62
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 431.30359
AFFX-BioB-M_at 761.37256
AFFX-BioB-3_at 731.40991
AFFX-BioC-5_at 1618.36963
AFFX-BioC-3_at 1110.56812
AFFX-BioDn-5_at 2822.6582
AFFX-BioDn-3_at 5548.96484
AFFX-CreX-5_at 8075.42285
AFFX-CreX-3_at 8924.72168
AFFX-DapX-5_at 42.04125
AFFX-DapX-M_at 5.75603
AFFX-DapX-3_at 18.903
AFFX-LysX-5_at 14.2783
AFFX-LysX-M_at 4.88431
AFFX-LysX-3_at 9.73203
AFFX-PheX-5_at 15.62058
AFFX-PheX-M_at 15.79715
AFFX-PheX-3_at 25.26746
AFFX-ThrX-5_at 19.4474
AFFX-ThrX-M_at 18.24244

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550837.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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