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Sample GSM550838 Query DataSets for GSM550838
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 36
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 60
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 753.93256
AFFX-BioB-M_at 1112.02441
AFFX-BioB-3_at 1058.98816
AFFX-BioC-5_at 2003.38318
AFFX-BioC-3_at 1548.65381
AFFX-BioDn-5_at 2966.52661
AFFX-BioDn-3_at 5314.96729
AFFX-CreX-5_at 8444.05859
AFFX-CreX-3_at 9024.32129
AFFX-DapX-5_at 27.31948
AFFX-DapX-M_at 11.15589
AFFX-DapX-3_at 16.59217
AFFX-LysX-5_at 15.5056
AFFX-LysX-M_at 2.20736
AFFX-LysX-3_at 13.08271
AFFX-PheX-5_at 15.39137
AFFX-PheX-M_at 10.17578
AFFX-PheX-3_at 21.84982
AFFX-ThrX-5_at 15.05675
AFFX-ThrX-M_at 20.16176

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550838.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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