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Sample GSM550839 Query DataSets for GSM550839
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 16
Sample type RNA
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 70
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 530.96234
AFFX-BioB-M_at 850.36139
AFFX-BioB-3_at 857.66437
AFFX-BioC-5_at 1809.4906
AFFX-BioC-3_at 1287.59509
AFFX-BioDn-5_at 2928.95386
AFFX-BioDn-3_at 5373.76855
AFFX-CreX-5_at 7389.93896
AFFX-CreX-3_at 8705.58984
AFFX-DapX-5_at 36.03735
AFFX-DapX-M_at 7.74055
AFFX-DapX-3_at 14.55776
AFFX-LysX-5_at 15.32572
AFFX-LysX-M_at 12.78337
AFFX-LysX-3_at 4.4084
AFFX-PheX-5_at 16.48613
AFFX-PheX-M_at 15.94536
AFFX-PheX-3_at 29.84414
AFFX-ThrX-5_at 12.67966
AFFX-ThrX-M_at 14.47061

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.

Supplementary file Size Download File type/resource
GSM550839.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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