NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM550842 Query DataSets for GSM550842
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 38
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 71
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 475.4892
AFFX-BioB-M_at 761.04498
AFFX-BioB-3_at 710.28387
AFFX-BioC-5_at 1565.71167
AFFX-BioC-3_at 1157.46094
AFFX-BioDn-5_at 2762.98047
AFFX-BioDn-3_at 4814.62451
AFFX-CreX-5_at 7093.10889
AFFX-CreX-3_at 8520.125
AFFX-DapX-5_at 51.43033
AFFX-DapX-M_at 9.88704
AFFX-DapX-3_at 22.87235
AFFX-LysX-5_at 11.47037
AFFX-LysX-M_at -0.01903
AFFX-LysX-3_at 10.11136
AFFX-PheX-5_at 14.33129
AFFX-PheX-M_at 14.5197
AFFX-PheX-3_at 26.96752
AFFX-ThrX-5_at 16.23055
AFFX-ThrX-M_at 12.54623

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550842.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap