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Sample GSM550846 Query DataSets for GSM550846
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 39
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 71
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 646.59369
AFFX-BioB-M_at 980.88678
AFFX-BioB-3_at 917.24438
AFFX-BioC-5_at 1851.90637
AFFX-BioC-3_at 1419.37317
AFFX-BioDn-5_at 3166.69531
AFFX-BioDn-3_at 5142.6626
AFFX-CreX-5_at 7936.28857
AFFX-CreX-3_at 8867.22852
AFFX-DapX-5_at 22.46317
AFFX-DapX-M_at 6.96441
AFFX-DapX-3_at 19.61993
AFFX-LysX-5_at 9.44785
AFFX-LysX-M_at 3.46153
AFFX-LysX-3_at 6.05718
AFFX-PheX-5_at 11.82031
AFFX-PheX-M_at 14.19137
AFFX-PheX-3_at 29.1205
AFFX-ThrX-5_at 6.28474
AFFX-ThrX-M_at 16.82503

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550846.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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