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Sample GSM550849 Query DataSets for GSM550849
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 40
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 71
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1151.85449
AFFX-BioB-M_at 1469.52148
AFFX-BioB-3_at 1988.79333
AFFX-BioC-5_at 3853.05103
AFFX-BioC-3_at 2676.39893
AFFX-BioDn-5_at 5178.25635
AFFX-BioDn-3_at 8877.74121
AFFX-CreX-5_at 11884.49121
AFFX-CreX-3_at 13834.84473
AFFX-DapX-5_at 12.58165
AFFX-DapX-M_at 16.26027
AFFX-DapX-3_at 9.72597
AFFX-LysX-5_at 11.5863
AFFX-LysX-M_at 10.65017
AFFX-LysX-3_at 67.4995
AFFX-PheX-5_at 23.13766
AFFX-PheX-M_at 30.45973
AFFX-PheX-3_at 30.37286
AFFX-ThrX-5_at 32.24074
AFFX-ThrX-M_at 27.82845

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550849.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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