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Sample GSM550850 Query DataSets for GSM550850
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 41
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 70
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1427.62598
AFFX-BioB-M_at 2166.75684
AFFX-BioB-3_at 2169.51367
AFFX-BioC-5_at 3481.85522
AFFX-BioC-3_at 2795.44092
AFFX-BioDn-5_at 4969.67334
AFFX-BioDn-3_at 7647.73291
AFFX-CreX-5_at 10568.69434
AFFX-CreX-3_at 11330.50781
AFFX-DapX-5_at 50.62524
AFFX-DapX-M_at 8.35928
AFFX-DapX-3_at 40.90572
AFFX-LysX-5_at 14.33874
AFFX-LysX-M_at 2.81851
AFFX-LysX-3_at 11.11794
AFFX-PheX-5_at 17.75977
AFFX-PheX-M_at 13.57109
AFFX-PheX-3_at 29.56482
AFFX-ThrX-5_at 8.38058
AFFX-ThrX-M_at 31.99298

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550850.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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