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Sample GSM550854 Query DataSets for GSM550854
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 22
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 66
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1020.44464
AFFX-BioB-M_at 1466.23083
AFFX-BioB-3_at 1380.92053
AFFX-BioC-5_at 2481.23975
AFFX-BioC-3_at 1942.83411
AFFX-BioDn-5_at 3675.81836
AFFX-BioDn-3_at 5911.56348
AFFX-CreX-5_at 7976.27686
AFFX-CreX-3_at 9302.06445
AFFX-DapX-5_at 31.32167
AFFX-DapX-M_at 14.91148
AFFX-DapX-3_at 21.38952
AFFX-LysX-5_at 16.70421
AFFX-LysX-M_at 1.22969
AFFX-LysX-3_at 8.05236
AFFX-PheX-5_at 15.18453
AFFX-PheX-M_at 14.3979
AFFX-PheX-3_at 25.9824
AFFX-ThrX-5_at 1.66383
AFFX-ThrX-M_at 19.06103

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550854.CEL.gz 4.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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