NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM550858 Query DataSets for GSM550858
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 24
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 69
gender: F
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 908.62811
AFFX-BioB-M_at 1337.79321
AFFX-BioB-3_at 1263.60791
AFFX-BioC-5_at 2319.2002
AFFX-BioC-3_at 1861.18079
AFFX-BioDn-5_at 3676.04663
AFFX-BioDn-3_at 5660.77881
AFFX-CreX-5_at 8154.90869
AFFX-CreX-3_at 9272.73926
AFFX-DapX-5_at 47.91911
AFFX-DapX-M_at 10.88554
AFFX-DapX-3_at 9.86214
AFFX-LysX-5_at 13.05177
AFFX-LysX-M_at 14.6033
AFFX-LysX-3_at 4.9855
AFFX-PheX-5_at 11.45081
AFFX-PheX-M_at 18.29086
AFFX-PheX-3_at 45.29564
AFFX-ThrX-5_at 10.48218
AFFX-ThrX-M_at 18.808

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550858.CEL.gz 5.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap