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Sample GSM550860 Query DataSets for GSM550860
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 5
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 64
gender: F
gold stage: Stage IV
group: Severe
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 709.44513
AFFX-BioB-M_at 1054.62671
AFFX-BioB-3_at 978.83844
AFFX-BioC-5_at 1945.61816
AFFX-BioC-3_at 1517.39526
AFFX-BioDn-5_at 3319.35132
AFFX-BioDn-3_at 5558.2793
AFFX-CreX-5_at 8733.8584
AFFX-CreX-3_at 9943.54102
AFFX-DapX-5_at 45.30081
AFFX-DapX-M_at 7.11674
AFFX-DapX-3_at 23.61594
AFFX-LysX-5_at 14.2782
AFFX-LysX-M_at 3.08893
AFFX-LysX-3_at 4.93122
AFFX-PheX-5_at 21.76359
AFFX-PheX-M_at 16.73084
AFFX-PheX-3_at 28.22503
AFFX-ThrX-5_at 7.85186
AFFX-ThrX-M_at 12.88083

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550860.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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