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Sample GSM550861 Query DataSets for GSM550861
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 47
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 64
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 684.70654
AFFX-BioB-M_at 1008.67926
AFFX-BioB-3_at 883.18243
AFFX-BioC-5_at 1977.78308
AFFX-BioC-3_at 1510.03345
AFFX-BioDn-5_at 3490.65747
AFFX-BioDn-3_at 5653.86621
AFFX-CreX-5_at 9031.89258
AFFX-CreX-3_at 9965.31543
AFFX-DapX-5_at 45.33036
AFFX-DapX-M_at 11.46593
AFFX-DapX-3_at 18.04406
AFFX-LysX-5_at 12.58295
AFFX-LysX-M_at 10.59384
AFFX-LysX-3_at 3.80256
AFFX-PheX-5_at 13.35986
AFFX-PheX-M_at 15.47005
AFFX-PheX-3_at 32.46429
AFFX-ThrX-5_at 12.58054
AFFX-ThrX-M_at 9.28776

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550861.CEL.gz 5.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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