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Sample GSM550870 Query DataSets for GSM550870
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 30
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 59
gender: F
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39323
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 546.25635
AFFX-BioB-M_at 869.54572
AFFX-BioB-3_at 791.26892
AFFX-BioC-5_at 1558.39392
AFFX-BioC-3_at 1223.25134
AFFX-BioDn-5_at 2829.03589
AFFX-BioDn-3_at 4987.83594
AFFX-CreX-5_at 7740.00586
AFFX-CreX-3_at 8385.34863
AFFX-DapX-5_at 28.08043
AFFX-DapX-M_at 6.88611
AFFX-DapX-3_at 21.63867
AFFX-LysX-5_at 9.57125
AFFX-LysX-M_at 5.64753
AFFX-LysX-3_at 7.01308
AFFX-PheX-5_at 18.9015
AFFX-PheX-M_at 14.85833
AFFX-PheX-3_at 27.73807
AFFX-ThrX-5_at 13.11606
AFFX-ThrX-M_at 16.14445

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550870.CEL.gz 5.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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