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Sample GSM550872 Query DataSets for GSM550872
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 32
Sample type RNA
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 72
gender: F
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 530.61713
AFFX-BioB-M_at 842.9245
AFFX-BioB-3_at 784.84161
AFFX-BioC-5_at 1740.36963
AFFX-BioC-3_at 1250.42651
AFFX-BioDn-5_at 3157.49902
AFFX-BioDn-3_at 5399.58154
AFFX-CreX-5_at 8575.50391
AFFX-CreX-3_at 9470.21289
AFFX-DapX-5_at 34.79869
AFFX-DapX-M_at 7.71443
AFFX-DapX-3_at 18.17374
AFFX-LysX-5_at 9.21602
AFFX-LysX-M_at 10.30305
AFFX-LysX-3_at 18.01458
AFFX-PheX-5_at 16.8211
AFFX-PheX-M_at 16.56171
AFFX-PheX-3_at 17.27327
AFFX-ThrX-5_at 5.27099
AFFX-ThrX-M_at 14.70195

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.

Supplementary file Size Download File type/resource
GSM550872.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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