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Sample GSM550873 Query DataSets for GSM550873
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 33
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 61
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 622.52356
AFFX-BioB-M_at 959.1554
AFFX-BioB-3_at 902.49988
AFFX-BioC-5_at 1894.05945
AFFX-BioC-3_at 1336.87817
AFFX-BioDn-5_at 3006.18213
AFFX-BioDn-3_at 5006.22461
AFFX-CreX-5_at 7550.53076
AFFX-CreX-3_at 8476.27539
AFFX-DapX-5_at 33.8094
AFFX-DapX-M_at 10.88046
AFFX-DapX-3_at 23.01345
AFFX-LysX-5_at 12.12374
AFFX-LysX-M_at 5.67704
AFFX-LysX-3_at 8.88893
AFFX-PheX-5_at 16.19025
AFFX-PheX-M_at 12.39492
AFFX-PheX-3_at 26.01676
AFFX-ThrX-5_at 2.16627
AFFX-ThrX-M_at 14.74459

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550873.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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