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Sample GSM550875 Query DataSets for GSM550875
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 6
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 68
gender: M
gold stage: Stage IV
group: Severe
plateid: 1
batch: A
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 879.01202
AFFX-BioB-M_at 1260.42615
AFFX-BioB-3_at 1163.87207
AFFX-BioC-5_at 2468.98462
AFFX-BioC-3_at 1825.56323
AFFX-BioDn-5_at 3886.10596
AFFX-BioDn-3_at 5906.73926
AFFX-CreX-5_at 8262.55371
AFFX-CreX-3_at 9191.32129
AFFX-DapX-5_at 55.24843
AFFX-DapX-M_at 8.18988
AFFX-DapX-3_at 31.35401
AFFX-LysX-5_at 12.69529
AFFX-LysX-M_at 6.90419
AFFX-LysX-3_at 7.12703
AFFX-PheX-5_at 15.33823
AFFX-PheX-M_at 12.12013
AFFX-PheX-3_at 23.99322
AFFX-ThrX-5_at 3.08129
AFFX-ThrX-M_at 23.71934

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550875.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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