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Sample GSM550878 Query DataSets for GSM550878
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 37
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 66
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 575.14911
AFFX-BioB-M_at 837.22461
AFFX-BioB-3_at 747.58228
AFFX-BioC-5_at 539.89917
AFFX-BioC-3_at 379.95737
AFFX-BioDn-5_at 3685.99878
AFFX-BioDn-3_at 5405.86182
AFFX-CreX-5_at 9246.6416
AFFX-CreX-3_at 9904.3457
AFFX-DapX-5_at 23.83278
AFFX-DapX-M_at 2.91036
AFFX-DapX-3_at 19.70537
AFFX-LysX-5_at 7.54602
AFFX-LysX-M_at 2.07438
AFFX-LysX-3_at 5.03152
AFFX-PheX-5_at 12.29128
AFFX-PheX-M_at 14.42823
AFFX-PheX-3_at 17.18308
AFFX-ThrX-5_at 9.69951
AFFX-ThrX-M_at 17.82005

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550878.CEL.gz 5.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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