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Sample GSM550879 Query DataSets for GSM550879
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 38
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 58
gender: F
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39315
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 760.11133
AFFX-BioB-M_at 1167.46912
AFFX-BioB-3_at 1070.05725
AFFX-BioC-5_at 767.05914
AFFX-BioC-3_at 593.03552
AFFX-BioDn-5_at 3866.948
AFFX-BioDn-3_at 5573.72656
AFFX-CreX-5_at 8696.04102
AFFX-CreX-3_at 9508.46777
AFFX-DapX-5_at 41.92294
AFFX-DapX-M_at 11.16339
AFFX-DapX-3_at 17.68835
AFFX-LysX-5_at 11.25157
AFFX-LysX-M_at 1.26409
AFFX-LysX-3_at 11.46777
AFFX-PheX-5_at 8.07706
AFFX-PheX-M_at 10.82771
AFFX-PheX-3_at 28.58371
AFFX-ThrX-5_at 10.75821
AFFX-ThrX-M_at 9.18385

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550879.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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