|
| Status |
Public on May 04, 2022 |
| Title |
control (KC1-G36-H1242), total RNA |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMC
Sex: Male
disease state: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from PBMC cell using TRIzol® RNA Isolation Reagents (Life technologies, Carlsbad, CA) based method. The quantity and quality of the RNA were evaluated using Agilent Nono Kit and assessing the RNA integrity number (RIN >7). One mg of total RNA was processed for preparing mRNA sequencing library using MGIEasy RNA Directional Library Prep Kit (MGI) according to manufacturer’s instruction.
RNA libraries were prepared for sequencing using standard MGI protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Data processing |
read trimming: skewer
mapping: STAR
quantification: Cuffquant
Genome_build: hg38
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Aug 07, 2021 |
| Last update date |
May 04, 2022 |
| Contact name |
Su-Jin Baek |
| Organization name |
KOREA INSTITUTE of ORIENTAL MEDICINE
|
| Street address |
1672, Yuseong-daero
|
| City |
Daejeon |
| ZIP/Postal code |
34054 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL30209 |
| Series (2) |
| GSE181646 |
Genome-wide DNA methylation proifling reveals candidate biomarkers and probable molecular mechanisms in metabolic syndrome [RNA-seq] |
| GSE181647 |
Genome-wide DNA methylation proifling reveals candidate biomarkers and probable molecular mechanisms in metabolic syndrome |
|
| Relations |
| BioSample |
SAMN20607198 |
| SRA |
SRX11670053 |
