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Sample GSM550880 Query DataSets for GSM550880
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 7
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 71
gender: M
gold stage: Stage IV
group: Severe
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1072.59924
AFFX-BioB-M_at 1512.52588
AFFX-BioB-3_at 1061.48059
AFFX-BioC-5_at 1265.07812
AFFX-BioC-3_at 937.00067
AFFX-BioDn-5_at 4147.12012
AFFX-BioDn-3_at 6716.48242
AFFX-CreX-5_at 9887.56152
AFFX-CreX-3_at 12081.65332
AFFX-DapX-5_at 50.9344
AFFX-DapX-M_at 18.2449
AFFX-DapX-3_at 40.11799
AFFX-LysX-5_at 20.20126
AFFX-LysX-M_at 29.68551
AFFX-LysX-3_at 12.96633
AFFX-PheX-5_at 18.73476
AFFX-PheX-M_at 19.8181
AFFX-PheX-3_at 25.119
AFFX-ThrX-5_at 8.78676
AFFX-ThrX-M_at 12.20993

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550880.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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