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Sample GSM550881 Query DataSets for GSM550881
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 8
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 72
gender: M
gold stage: Stage IV
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 936.95312
AFFX-BioB-M_at 1343.65491
AFFX-BioB-3_at 1237.86023
AFFX-BioC-5_at 948.25427
AFFX-BioC-3_at 680.78412
AFFX-BioDn-5_at 4536.0293
AFFX-BioDn-3_at 6537.27979
AFFX-CreX-5_at 9801.14062
AFFX-CreX-3_at 10683.94141
AFFX-DapX-5_at 37.52573
AFFX-DapX-M_at 4.06063
AFFX-DapX-3_at 18.75731
AFFX-LysX-5_at 7.09005
AFFX-LysX-M_at 7.37622
AFFX-LysX-3_at 7.58425
AFFX-PheX-5_at 11.66181
AFFX-PheX-M_at 13.89174
AFFX-PheX-3_at 35.34821
AFFX-ThrX-5_at 7.61248
AFFX-ThrX-M_at 26.51577

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550881.CEL.gz 4.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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