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Sample GSM550884 Query DataSets for GSM550884
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 10
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 61
gender: M
gold stage: Stage IV
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 606.54803
AFFX-BioB-M_at 892.27869
AFFX-BioB-3_at 812.96381
AFFX-BioC-5_at 605.27454
AFFX-BioC-3_at 393.21924
AFFX-BioDn-5_at 3527.80737
AFFX-BioDn-3_at 5542.94775
AFFX-CreX-5_at 8700.62109
AFFX-CreX-3_at 9943.46484
AFFX-DapX-5_at 37.44164
AFFX-DapX-M_at 3.91294
AFFX-DapX-3_at 25.51969
AFFX-LysX-5_at 9.94723
AFFX-LysX-M_at 6.94033
AFFX-LysX-3_at 4.64514
AFFX-PheX-5_at 13.68517
AFFX-PheX-M_at 7.54131
AFFX-PheX-3_at 21.73101
AFFX-ThrX-5_at 1.16139
AFFX-ThrX-M_at 12.48212

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550884.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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