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Sample GSM550886 Query DataSets for GSM550886
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 53
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 74
gender: M
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 587.26166
AFFX-BioB-M_at 837.74878
AFFX-BioB-3_at 761.87909
AFFX-BioC-5_at 542.21936
AFFX-BioC-3_at 386.60513
AFFX-BioDn-5_at 3456.53442
AFFX-BioDn-3_at 5274.92627
AFFX-CreX-5_at 8270.51074
AFFX-CreX-3_at 9080.81348
AFFX-DapX-5_at 47.62813
AFFX-DapX-M_at 9.66086
AFFX-DapX-3_at 20.59717
AFFX-LysX-5_at 10.65136
AFFX-LysX-M_at 13.8425
AFFX-LysX-3_at 11.51401
AFFX-PheX-5_at 13.59841
AFFX-PheX-M_at 10.87872
AFFX-PheX-3_at 26.2594
AFFX-ThrX-5_at 12.0319
AFFX-ThrX-M_at 7.5972

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550886.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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