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Sample GSM550890 Query DataSets for GSM550890
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 41
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 64
gender: F
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 662.73688
AFFX-BioB-M_at 928.19611
AFFX-BioB-3_at 841.93842
AFFX-BioC-5_at 637.9342
AFFX-BioC-3_at 473.80206
AFFX-BioDn-5_at 3626.93457
AFFX-BioDn-3_at 5323.84375
AFFX-CreX-5_at 8233.85254
AFFX-CreX-3_at 9192.90527
AFFX-DapX-5_at 46.17978
AFFX-DapX-M_at 8.54709
AFFX-DapX-3_at 34.34189
AFFX-LysX-5_at 8.61853
AFFX-LysX-M_at 4.44854
AFFX-LysX-3_at 6.73342
AFFX-PheX-5_at 12.42474
AFFX-PheX-M_at 11.89875
AFFX-PheX-3_at 22.45389
AFFX-ThrX-5_at 13.49753
AFFX-ThrX-M_at 14.59855

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550890.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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